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Leica M Stereo- microscopes User Manual.
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3 Leica M Stereomicroscopes User Manual Dear User Thank you for choosing our products. W e hope that you will en- joy the quality and performance of Leica Microsystems products. In developing our instruments, we have placed great emphasis on simple, self-explanatory directions.
Leica M Stereomicroscopes User Manual T able of contents 4 Page Overview Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5 Page Illuminators T ransformers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 Incident lamp 6 V/10 W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Incident lamp 6 V/20 W . . . . . .
Read the user manual and safety instructions before starting up. The Leica MS5, MZ6, MZ7 5 , MZ9 5 , MZ12 5, MZ16 and MZ16 A stereomicro- scopes are optical instruments which use magnification for improved visualization of objects, their details and specimens from technology and natural sciences.
7 Leica M Stereomicroscopes – Safety regulations Repairs may only be carried out by Leica-trained service tech- nicians. Only original Leica spare parts may be used. • Ensure that personnel who use the instrument have read and understood this user manual and in particular the safety instructions.
Liquids Power cables Cable routing Opening the instrument Supply voltage Connections Changing bulbs 8 Leica M Stereomicroscopes – Safety regulations Electrically-operated items The safety regulation.
The following must be observed if Leica products are build into third-party products: The manufacturer of the complete system or the person putting it on the market is responsible for adhering to applicable safety regulations, laws and guidelines. Adhere to general and local regulations relating to accident prevention and environmental protection.
Stereomicroscope 1 Magnification changer 2 Engaging ratchet positions 3a Focusing drive 3b Coarse and fine focusing Larger knob: Coarse focusing, Smaller knob: Fine focusing. 3c Adjustable ring Changes the ease of movement of the coarse/fine drive. 4 Clamping screw Holds the optics carrier in the microscope carrier .
11 Leica M Stereomicroscopes – Overview Leica design by Ernest Igl/Christophe Apothéloz Leica MZ9 5 7 5 4 6 2 1 3c 3b 8 Leica MZ7 5 7 5 4 6 2 1 3c 3b 8 Leica MZ12 5 7 5 10 4 6 2 1 3c 3b 8 Leica MZ1.
Magnification changer • Zoom means continuous change of magnification. Ratchets can be engaged for certain magnifications (see below). • For zooming with MZ16 A, see the user manual M2-116-0. MS5: 5 steps MZ6: 6:1 zoom MZ7 5 : 7.9:1 zoom MZ9 5 : 9.
MS5, MZ6: Display of magnification factors from 0.63–4. MZ7 5 : Display of magnification factors from 0.63–5. Corresponding scales for other objective and eyepiece combi- nations can be affixed to the rotary knobs (page 34). MZ9 5 : Display of magnification factors from 0.
14 Leica M Stereomicroscopes – Operation Ergonomics, viewing height • Pay great attention when setting up your stereomicroscope. Y ou will only benefit fully from the outstanding optical and ergonomic advantages of the instrument if you set it up precisely in accordance with the directions already given.
– ErgoWedge™ 5° – 25° – ErgoWedge™ ±15° – ErgoModule™ 50 mm – The ErgoT ube™ 45° has eyepiece tubes which are 65 mm longer than the standard ones. Magnification factor 1.6 × . MZ16 A: The magnification factor can be toggled on and off using the OPT key .
Y ou do not wear glasses and prefer close contact with 10 × /21B wide-field eyepieces for persons wearing glasses: 왘 Holding the diopter ring, turn the eyecup counterclockwise until it is released. 왘 Pull the eyecup upwards. 왘 Holding the diopter ring, turn the eyecup clockwise.
17 Leica M Stereomicroscopes – Operation 왘 Place the object beneath the objective. 왘 Engage the lowest magnification (page 12). 왘 Look into the eyepieces. 왘 Using the rotary knob, bring the object into focus. The coarse/fine drive supports up to 15 kg.
Microscope carrier • This microscope carrier permits only stereoscopic observation, using both beam paths. • The microscope carrier can be fitted to the drive housing in either of two positions (s.
The parallax-free imaging produces more precise results in photography , measuring and polarization. 왘 Shift the stereomicroscope to the left or the right position.
20 Leica M Stereomicroscopes – Operation Adjusting diopter settings and parfocality Leica stereomicroscopes are parfocally matched. This means that, once you have focused on a feature in the manner de- scribed, taking account of diopter settings, you can zoom the magnification up and down without refocusing.
4. Engage the highest magnification position. 5. Optimize the focus using the focusing drive. 6. Engage the lowest magnification position. 7. Do not look into the eyepieces. 왘 Rotate the eyepieces counterclockwise in the "+" direction as far as the stop (+5 diopters).
The components 1a Incident-light base with stage plate 1b T ransmitted-light base with glass stage plate 2a Side-faced column with drive housing, coarse/fine 2b Side-faced column with drive housing, 3.
23 Leica M Stereomicroscopes – Assembly Stand Incident-light stand 왘 Remove the stage plate. 왘 Insert 3 Allen screws into the underside of the baseplate and secure them to the bottom end of the side-faced column.
T ransmitted-light stand, bright field, 20 W Follow the safety instructions given on page 7. 왘 Fit the side-faced column to the transmitted-light base as described on page 23.
Use: – T wo 160 mA T fuses for voltages between 200–240 V . – T wo 315 mA T fuses for voltages between 100–120 V (see the section "Starting up" on the left). 왘 Disconnect the power cable from the stand. 왘 Pull out the fuse holder .
T ransmitted-light stand, bright and dark field The illumination of the transmitted-light stand bright and dark field consists of a fiber -optic light guide and a light source. Observe the safety regulations from the manufacturer of the light source. 왘 Unplug the fiber -optic light guide from the connection in the stand.
T ransmitted-light stand HL The column seat contains Allen screws. 왘 Remove the glass stage plate. 왘 Invert the transmitted-light base and place it on a table. 왘 Hold column against the column seat from underneath. 왘 Insert the Allen screws into the column from the top and tighten them.
Swinging-arm stand ESD The swinging-arm stand ESD is of conducting material (surface resistance <10 11 Ohm/squared, discharge time <2 seconds from 1,000 V to 100 V). T wo sockets for connecting ∅ 4 mm ground cables are provided on the base. 왘 Insert 4 Allen screws into the base from beneath and secure them to the end of the column.
The focusing knobs are located on the inclinable drive housing. Is the focus movement too loose or too tight? Does the outfit tend to slide downwards? Y ou can adjust the movement in accordance with t.
Large swinging-arm and table-clamp stands 왘 Use the clamp to secure the column to a table top 20 mm – 50 mm thick. 왘 Insert 4 Allen screws into the base from beneath and secure them to the end of the column. The safety ring secures the instrument and must always be positioned beneath the horizontal arm.
왘 Secure as described on page 34. 왘 Secure as described on page 34. Possible adjustments 왘 Release the clamping lever . 왘 T ilt the instrument. 왘 Retighten the clamping lever . 왘 Set large distances with the larger knob. 왘 Fine-focus with the smaller (concentric) knob.
10 445 614 MZ6 10 411 589 1x 10 422 562 1.5x 10 447 081 2x 10 473 832 0.8x 10 445 201 0.63x 10 422 563 0.5x 10 422 564 0.32x Achromat M1-105-MZ16 / 01.04 10 446 371 MZ7 5 10 446 272 MZ9 5 10 447 102 MZ16 10 447 103 MZ1 6 A 10 445 613 MS5 10 446 172 10 411 589 1x 10 422 562 1.
33 Leica M Stereomicroscopes User Manual Objective combinations C Compatible CA Compatible if used with intermediate ring (order separately) 0 Intermediate ring is recommended M Magnification increased by factor 1.25 × or higher * Remove intermediate ring (10 446 393); already included with MZ9 5 .
Microscope carrier The microscope carrier can be attached to the focusing drive in 2 positions: • For short working distances and for flat objects, use Position 1. Use this position also in conjunction with the attachment for vertical and oblique observation (see detailed in- structions).
35 Leica M Stereomicroscopes – Assembly Binocular tube 왘 Release the clamping screw . • If you have an accessory such as an ergo module (page 14), a coaxial illumi- nator (page 47) or a video/phototube (page 52), fit it now to the optics carrier .
Objective nosepiece • Suggested for Leica MZ16 and MZ16 A Please note: When using the objective nosepiece together with MS5, MZ6, MZ7 5 , MZ9 5 or MZ12 5 large fields of view at low magnifications are not fully illuminated. – Hold objectives during assembly and disassembly to prevent them from falling on the stage plate.
왘 Carefully turn and tighten the 2 × planapochromatic objective clockwise to the limit stop without applying excessive force. 왘 Raise the focusing drive with the drive heads to provide sufficient space for the 2 × planapochro- matic objective.
T ransformers Follow the safety instructions given on page 7. Leica transformers may only be opened by authorized service personnel, and the power cable must be disconnected before- hand. The transformers are not to be used outdoors. Regulating transformer The regulating transformer 5.
Y ou can prolong the life of your bulb if you: 왘 First switch off the lamp by turning the rotary knob into the black range 왘 and only then switch the transformer on or off. 왘 T urn on the power switch. The diode is illuminated. 왘 Use the rotary knob to adjust the brightness.
6 V/10 W incident lamp • The 6 V/10 W lamp is an inclined incident source for illuminating spatial objects • By using two lamps, you can soften or eliminate shadows. • At the nominal voltage (6 V), the color temperature is 2700 K. 왘 Release the clamping screw .
Observe the safety notes on page 7. Observe the notes with respect to the use of the trans- former on page 38. 왘 Connect the cable between the lamp housing and the transformer (page 38). 왘 Switch on the transformer and use the rotary knob to adjust the brightness (page 39).
42 Leica M Stereomicroscopes – Illuminations 6 V/20 W incident lamp • The 6 V/20 W lamp is an inclined incident source for illuminating spatial objects. • By using two lamps, you can soften or eliminate shadows. • At the nominal voltage (6 V), the color temperature is 3200 K, ideal for photography .
43 Leica M Stereomicroscopes – Illuminations Observe the safety notes on page 7. Observe the notes with respect to the use of the transformer on page 38. 왘 Connect the cable between the lamp housing and the trans- former (page 38). 왘 Switch on the transformer and use the rotary knob to adjust the brightness.
44 Leica M Stereomicroscopes – Illuminations Disconnect the lamp cable from the transformer . Never touch the bulb when it is hot. Y ou may sustain burns. • Do not touch the 6 V/20 W halogen bulb directly with your fin- gers, because finger marks can cause it to crack when heated.
45 Leica M Stereomicroscopes – Illuminations Leica L2 cold-light source The antistatic Leica L2 cold-light source is powerful, small, compact, inexpensive and suitable for simple observation tasks with the Leica MS5 and MZ6 stereo microscopes.
46 Leica M Stereomicroscopes – Illuminations Cold light source Leica CLS series The Leica CLS series is a high-performance line for high light intensity within the smallest possible space and flicker -free white light with the lowest possible thermal effect on the ob- jects.
47 Leica M Stereomicroscopes – Illuminations Supply (line) lamp 25W Follow the safety instructions given on page 7. 왘 Fit the lamp holder and lamp as described below (incident-light lamp 6 V/10 W). 왘 Connect the 25 W supply (line) lamp to the power supply .
Near -vertical illuminator • The near -vertical (approx. 5°) light beam illuminates depres- sions and cavities. • Shadowing by tools is eliminated. • This illuminator can only be used with the achromatic objec- tives having focal lengths of 100, 150, 175 and 200 mm.
49 Leica M Stereomicroscopes – Illuminations 왘 Attach the fiber -optic light guide on either the left or the right. T o switch on the cold-light illuminator , refer to the user manual provided with it. 왘 T urn the rotary knob. • A movable prism directs the light from the left or right outlet onto the object.
T ubes • All accessory tubes which fit between the optics carrier and the binocular tube are fitted in the same manner . • In the dovetail ring of the optics carrier is a locating screw which must engage the notch in the accessory (page 35). This is best accomplished by moving the accessory slightly from side to side.
Drawing tube • The drawing tube is for both left-handers and right-handers. Fit the mirror on the left-hand or right-hand side of the optics carrier . 왘 Fit the drawing tube between optics car- rier and binocular tube as described on page 50. 왘 Place a sheet of paper beneath the mirror .
Video/phototubes 52 Leica M Stereomicroscopes – Accessories Leica MPS30 / MPS60 photoautomats 10 445 927 10 445 924 50% 10 446 229 100% 10 446 121 8x 10 446 131 10x 10 446 132 16x 11 541 038 0.
• Video/phototubes are designed to support modular photomicrography systems, and Leica digital, video, film or SLR cameras. • The modular photomicrographic systems and the Leica digi- tal cameras are detailed in a separate user manual, along with instructions for their assembly and use.
T rinocular video/phototube 100% Fitting: See "T rinocular video/phototube 50%". 왘 Observation: Push lever in. 왘 Photography: Pull out lever to the left. • 100% in both eyepieces. • 100% in the left eyepiece • 100% in the video/photo beam path.
Video/phototube HD50 왘 Use the video/phototube HD50 between the optics carrier and the binocular tube (page 50). 왘 Observation: Push lever in. 왘 Documentation: Pull lever out.
Attachment for vertical and oblique observation • The attachment for vertical and oblique observation enables spatial objects to be observed from above, and also from all around at an angle of 45°. • The microscope carrier must be fitted in the lower position 1 (page 34).
Stages The ∅ 120 mm stages can be used in those incident and trans- mitted-light stands which have a ∅ 120 mm port. The gliding stage can be moved in all directions and rotated completely . 왘 Place the ∅ 120 mm gliding stage in the ∅ 120 mm port and secure it with the eccentric lever .
58 Leica M Stereomicroscopes – Notes T ips and hints: What to do if … Remedy: • Set the interpupillary distance correctly (page 15). • Locate the eyepoint (page 15). Remedy: • Fit the eyepieces correctly (page 35). • Adjust your dioptric settings exactly as described (page 20).
59 Leica M Stereomicroscopes – Notes Care, maintenance W e will show you how to look after your microscope and how to clean it. Y ou are working with a high-precision, high-performance instrument. Accordingly , we guarantee the quality of each of our instru- ments.
60 Leica M Stereomicroscopes – Notes Cleaning Dust and dirt will affect the quality of your results. 왘 Put a dust cover over the instrument during breaks in work. 왘 Use dust caps to protect tube openings, tubes without eyepieces, and eyepieces. 왘 Remove dust with a pneumatic rubber bulb or with a soft brush.
61 Leica M Stereomicroscopes – Notes Calculation M O Magnification of objective M E Magnification of eyepiece z Magnification changer position q T ube factor , e.
62 Leica M Stereomicroscopes User Manual Optical data of Leica MS5, MZ6 Objectives Working distance (mm) Field diameter (mm) T otal magnification 0.63 25.2 9.5 31.5 7.6 50.4 4.8 20.2 11.9 15.8 15.2 7.9 30.4 12.6 19 37.6 6.4 0.8 32 7.5 40 6 64 3.8 25.6 9.
63 Leica M Stereomicroscopes User Manual Objectives Working distance (mm) 0.63 16 14.9 10.4 23 0.8 20.4 11.8 13.2 18.2 1 25.5 9.4 16.5 14.5 1.25 31.8 7.5 20.6 11.7 1.6 40.8 5.9 26.3 9 2 51 4.7 32.9 7.3 2.5 63.7 3.8 41.2 5.8 3.2 81.5 2.9 52.7 4.6 4 101.
64 Leica M Stereomicroscopes User Manual Optical data of Leica MZ7 5 , MZ9 5 Objectives 40 × /6B 0.63 25.2 9.5 31.5 7.6 50.4 4.8 20.2 11.9 15.8 15.2 7.9 30.4 12.6 19 37.6 6.4 0.8 32 7.5 40 6 64 3.8 25.6 9.4 20 12 10 24 16 15 47.8 5 1 40 6 50 4.8 80 3 32 7.
65 Leica M Stereomicroscopes User Manual Objectives 40 × /6B 0.63 16 14.9 10.4 23 0.8 20.4 11.8 13.2 18.2 1 25.5 9.4 16.5 14.5 1.25 31.8 7.5 20.6 11.7 1.6 40.8 5.9 26.3 9 2 51 4.7 32.9 7.3 2.5 63.7 3.8 41.2 5.8 3.2 81.5 2.9 52.7 4.6 4 101.9 2.4 65.8 3.
66 Leica M Stereomicroscopes User Manual Optical data of Leica MZ12 5 , MZ16, MZ16 A Eyepieces Working distance (mm) Objectives 0.71 28.4 8.4 14.2 16.9 17.9 13.4 22.7 10.5 45.4 5.3 56.8 4.2 0.8 32 7.5 16 15 20.5 11.7 25.6 9.4 51.2 4.7 64 3.8 1 40 6 20 12 25.
67 Leica M Stereomicroscopes User Manual MS5 1.0x Achromat MS5 Dimensions of Leica MS5 with incident-light stand with transmitted-light stand Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency .
68 Leica M Stereomicroscopes User Manual Dimensions of Leica MZ6 with incident-light stand with transmitted-light stand Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency .
69 Leica M Stereomicroscopes User Manual Dimensions of Leica MZ7 5 with incident-light stand with transmitted-light stand Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency .
70 Leica M Stereomicroscopes User Manual Dimensions of Leica MZ9 5 with incident-light stand with transmitted-light stand Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency .
71 Leica M Stereomicroscopes User Manual Dimensions of Leica MZ12 5 with incident-light stand with transmitted-light stand Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency .
1x Planapo 1x Planapo MZ16 MZ16 72 Leica M Stereomicroscopes User Manual Dimensions of Leica MZ16 with incident-light stand with transmitted-light stand Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency .
73 Leica M Stereomicroscopes User Manual Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency . Dimensions of Leica MZ16 A with incident-light stand with .
74 Leica M Stereomicroscopes User Manual T ype description T ransmitted light base 20 W Power supply ES 635 474 incl. potentiometer T ype of bulb Halogen bulb 6 V/20 W Socket Connection for one extern.
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