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Leica RM2245 Rotary Microtome Instruction Manual Leica RM2245 V1.4 English - 02/2007 Always keep this manual with the instrument. Read carefully before working with the instrument.
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3 Leica RM2245 The information, numerical data, notes and val- ue judgments contained in this manual repre- sent the current state of scientific knowledge and state-of-the-art technology as we under- stand it following thorough investigation in this field.
4 Instruction manual V 1.4 – 02/2007 TABLE OF CONTENTS 1. Important notes .......................................................................................................................................... 6 1.1 Symbols in the text and their meanings .
5 Leica RM 2245 TABLE OF CONTENTS 6.1.3 Fine-directional fixture for specimen clamps .............................................................................. .3 7 6.1.4 Quick clamping system ......................................................
6 Instruction manual V 1.4 – 02/2007 Function keys which must be pressed on the control panel are shown in the text in bold capital letters. 1. 1 Symbols in the text and their meanings 1.
7 Leica RM2245 2. Safety 2.1 Safety notes This instruction manual contains important instructions and information regarding the operational safety and maintenance of the instrument. The instruction manual is an important part of the product, which must be read carefully prior to startup and use and must always be kept near the instrument.
8 Instruction manual V 1.4 – 02/2007 2. Safety Warnings – Safety instructions / warning labels attached to the instrument • Safety instruction labels on the instrument marked with a warning tria.
9 Leica RM 2245 Warnings – Working at the instrument • Take care when handling microtome knives and disposable blades. The cutting edge is extremely sharp and can cause severe injury! • Always remove the knife / blade before detaching the knife holder from the instrument.
10 Instruction manual V 1.4 – 02/2007 2.3 Integrated safety devices 2. Safety Locking the handwheel There are two ways of locking the handwheel ( 12 ): Using the lever ( 3 ) on the right side of the micro- tome base plate, the handwheel can be braked in almost any position.
11 Leica RM2245 2. Safety Knife guard on the knife holder Each knife holder is equipped with a tightly mounted knife guard ( 8 , 9 ). This makes it possi- ble to cover completely the cutting edge in every knife or blade position.
12 Instruction manual V 1.4 – 02/2007 3.1 Overview – instrument components 3. Instrument components and specifications Leica RM2245 Knife guard on the knife holder Smooth-turning handwheel Lever of handwheel brake Handwheel locking mechanism Separate control panel Directional specimen holder fixture with quick clamping system Fig.
13 Leica RM2245 3. Instrument components and specifications Fig. 7 Vents Voltage selector switch with fuses Main switch Power connection Connecting cable for control panel Rear side of instrument Magnet for holding the wrench size 4 3.2 Instrument specifications The Leica RM2245 is a semi-motorized rotary microtome with a light-action handwheel.
14 Instruction manual V 1.4 – 02/2007 3.3 Technical data General Approvals: The inst rument-specific marks are located next to the identification label. Nominal supply voltages: 100 / 120 / 230 / 240 V AC ±10% Nominal frequency: 50/60 Hz Power draw: 70 VA Protective class ➀ ➀ ➀ ➀ ➀ :I Power fuses: 2 x T 1.
15 Leica RM 2245 Microtome Sectioning thickness setting: Section thickness setting range: 0.50 - 100 μm Setting values: from 0.50 μm - 5.0 μm in 0.5 μm increments from 5.0 - 20.0 μm in 1.0 μm increments from 20.0 - 60.0 μm in 5.0 μm increments from 60.
16 Instruction manual V 1.4 – 02/2007 4.1 Standard delivery The Leica RM2245 standard delivery includes: 1 Leica RM2245 basic instrument, without orientation ................. 0501 38178 1 handwheel, complete ........................................
17 Leica RM2245 Setting up the instrument Do not transport the instrument by holding it on the handwheel shaft, the object head or the cover! • By holding the instrument ( 5 ) at the front by the baseplate, and at the rear by the recessed grip (Fig.
18 Instruction manual V 1.4 – 02/2007 4. Startup The feather key ( 4 ) is loosely placed in the handwheel shaft ( 1 ) and fixed in place with a tie-rap during transport. • Remove the cable tie. Caution! Make sure not to lose the feather key! • Place the handwheel ( 2 ) on the handwheel shaft ( 1 ) as shown.
19 Leica RM2245 4. Startup 22 25 24 21 23 The voltage selector is located above the main power switch, on the left-rear side of the instru- ment ( Fig. 10 ). The voltage setting is displayed in the viewing window ( 22 ). • Insert a small screwdriver into the cutout ( 25 ) and carefully pry out the insert.
20 Instruction manual V 1.4 – 02/2007 4. Startup 4.5 Switching on the instrument Exposure to extreme temperature changes and high air humidity may cause condensation to form inside the instrument.
21 Leica RM 2245 5. Operation 5.1 Operating elements and their functions The operating functions of the microtome are divided between a control panel and a display unit on the microtome. A control panel on the instrument displays the current operating mode as well as various settings.
22 Instruction manual V 1.4 – 02/2007 5. Operation Three-digit display for section thickness/ trimming section thickness LED TRIM (green) Lights up when trim- ming mode is activated LED SECT (green) Lights up when sectioning mode is activated 5.
23 Leica RM2245 5. Operation 5.1.3 Display and control elements Three-digit display This display is located both on the instrument and on the control panel. If the SECT LED is lit up, the display shows the section thickness setting in μm. If the TRIM LED is lit up, the display shows the trimming section thickness setting in μm.
24 Instruction manual V 1.4 – 02/2007 In sectioning mode the user can select between STEP function (step- by-step specimen feed) and continuous specimen feed. When continuous feed is selected, the coarse feed buttons have the same func- tions as in trimming mode.
25 Leica RM2245 5. Operation • To change the display mode, push MENU MODE until the LED of the desired mode is illuminated. • Press CLEAR to reset section thickness sum or section number.
26 Instruction manual V 1.4 – 02/2007 Configuring the retraction settings • To call up the retraction settings, press the MENU MODE and CLEAR buttons simultaneously. • The current set value is displayed as a three-digit number in the four- digit display (e.
27 Leica RM2245 Indication of remaining horizontal feed The visible and audible remaining feed indica- tion feature informs the user during trimming and sectioning when a remaining feed of ap- proximately 1 mm is available before the front limit is reached.
28 Instruction manual V 1.4 – 02/2007 In the quick clamping device of the directional specimen holder fixture, all specimen clamps available as optional accessories can be used (implemented).
29 Leica RM2245 Fig. 18 Fig. 19 33 34 Important! Never turn the screw more than 1 / 2 turn at a time. 5. Operation Fine adjustment of the force balance If another accessory of a different weight is mounted on the object head ( 33 ), you must check whether it is necessary to readjust the force balance.
30 Instruction manual V 1.4 – 02/2007 Setting up the knife holder base • Release the clamping lever ( 50 ) by rotating it counterclockwise. • Insert the knife holder base ( 51 ) using the notch ( 52 ) on the bottom into the T-piece ( 55 ) of the microtome base plate ( 53 ).
31 Leica RM2245 5. Operation There are two versions of the specimen holder, one with and one without specimen orientation, which are interchangeable. The object orientation allows for simple position correction of the specimen surface when the specimen is clamped into place.
32 Instruction manual V 1.4 – 02/2007 5. Operation • The index marks (0 °, 5 ° and 10 °) for adjust- ment of the clearance angle ( 59.1 ) are located on the right side of the knife holder ( 57 ).
33 Leica RM 2245 5. Operation 5.5 Clamping the specimen Always clamp the specimen block BEFORE clamping the knife. Lock the handwheel and cover the knife edge with the knife guard prior to any manipul.
34 Instruction manual V 1.4 – 02/2007 5. Operation 5.7 Cutting into the specimen (trimming) • Use the TRIM/SECT key to select the trim mode. • Set the desired trim section thickness. • Release the handwheel lock and the brake. •I n TRIM mode, use the coarse feed buttons to move the sample against the knife/blade.
35 Leica RM 2245 5.9 Changing the specimen or interrupting sectioning Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of knife or object head, as well as pri.
36 Instruction manual V 1.4 – 02/2007 6.1 Assembly for fixture for specimen clamps Depending upon the purchase order, the basic instrument is delivered with the directional or rigid fixture for specimen clamps which must be assembled first. All specimen clamps available as accessories can be used in both fixtures for specimen clamps.
37 Leica RM2245 6. Optional accessories 6.1.3 Fine-directional fixture for specimen clamps • Before the fine-directional fixture for specimen clamps can be mounted, loosen 4 screws ( 10 ) (Allen key size 3) and carefully remove the fixture for specimen clamps from the baseplate ( 9 ).
38 Instruction manual V 1.4 – 02/2007 6.1.4 Quick clamping system It is used as specimen holder for use with the fine-directional fixture for specimen clamps with zero point indicators or the directional fixture for specimen clamps. • Screw the 4 screws ( 13 ) into bore A with an Allen key size 2.
39 Leica RM2245 6. Optional accessories 6.2.1 Standard specimen clamp The standard specimen clamps are available in two sizes: 40 x 40 mm and 50 x 55 mm. They are designed for direct clamping of rectangular blocks. In addition, they accommo- date the foil clamps.
40 Instruction manual V 1.4 – 02/2007 6. Optional accessories Clamping of foil pieces • Move the movable jaw ( 74 ) to the right as required by turning the set screw with an Allen key size 4 ( 71 ). • Place the foil ( 72 ) between the movable jaw ( 74 ) and the fixed jaw ( 73 ).
41 Leica RM2245 • To open the jaws ( 78 ) and ( 79 ) lightly loosen the 3 screws ( 81 ) with an Allen key size 4 ( 71 ). • Insert the foil ribbon ( 72 ) from behind to po- sition it between the movable jaw ( 79 ) and the fixed jaw ( 78 ).
42 Instruction manual V 1.4 – 02/2007 65 The universal cassette clamps (UCC) are designed for horizontal or vertical clamping of all kinds of commercial cassettes. • Push the lever ( 60 ) forwards. • Mount the cassette ( 65 ) horizontally or vertically as required.
43 Leica RM2245 Assembly of the super mega-cassette clamp The super mega-cassette clamp should preferably be used with the rigid fixture for specimen clamps.
44 Instruction manual V 1.4 – 02/2007 • To mount the required insert ( 89.1-3 ) turn the clamping ring ( 90 ) counterclockwise and remove it. • Place the required insert into the tension ring ( 90 ) and screw the tension ring onto the thread ( 91 ) by turning it clockwise.
45 Leica RM2245 6. Optional accessories 6.3 Knife holder base and knife holder Repositioning the knife holder base The one-piece knife holder base (rigid) ( 51 ) can be moved forwards and backwards on the microtome base plate. This vertical displacement allows bringing the knife holder into the optimal cutting position in relation to the specimen.
46 Instruction manual V 1.4 – 02/2007 6. Optional accessories Prior to inserting the blade, both knife holder and knife holder base must have been installed on the instrument! Inserting the blades, knife holder E and E-TC • Fold knife guard ( 9 ) downward.
47 Leica RM2245 6. Optional accessories Lateral displacement (only for knife holder E) The lateral movement feature of the knife holder base enables the use of the entire length of the blade or knife, eliminating the need for re- adjusting the knife holder.
48 Instruction manual V 1.4 – 02/2007 Knife holder E with water bath for narrow-band and broad-band blades 6. Optional accessories Fig. 42 Knife holder E with water bath is available for both narrow-band and broad-band blades. The knife guard on knife holder E consists of a red foldaway handle ( 9 ).
49 Leica RM 2245 6.3.3 Knife holder N/NZ The knife holders N and NZ are appropriate for standard steel and tungsten carbide knives, profile c and d, up to 16 cm long. The integrated height adjustment feature allows you to also use knives that have been resharpened numerous times.
50 Instruction manual V 1.4 – 02/2007 6. Optional accessories Inserting the knife • Rotate the knurled nuts ( 48 ) on the right and left of the knife holder forward in opposite directions, lowering the knife support bar to the lowest possible position, thus ensuring that the knife edge will not be damaged when inserting the knife.
51 Leica RM2245 6. Optional accessories The backlighting is inserted at the front into the one-piece knife holder base. • Remove the two screws ( 1 ) using a slotted screwdriver and then remove the cover plate ( 2 ). • Insert the backlighting ( 3 ) in the recess at the rear of the knife holder base.
52 Instruction manual V 1.4 – 02/2007 6. Optional accessories 6.7 Freezer pack Fig. 48 98 99 98 Fig. 47 6.6 Tray The freezer pack consists of the freezer plate ( 98 ) and the insulation jacket ( 99 ). It is for cooling (or keeping cold) paraffin specimens.
53 Leica RM2245 6.8 Universal microscope carrier 6. Optional accessories 1 - Baseplate with drilling (a) 2 - Vertical column with size 8 cap screw (b) and lock washer (c) 3 - Horizontal arm with cross.
54 Instruction manual V 1.4 – 02/2007 2. Attach vertical column • Insert the cap screw ( b ) into the hole of the baseplate from below. Place the lock washer ( c ) on the cap screw from above. • Thread the silver vertical column ( 2 ) onto the baseplate from above and tighten with the Allen key size 8.
55 Leica RM2245 6. Optional accessories 6.9 Magnifying lens Fig. 55 Fig. 57 Fig. 56 1 3 The magnifying lens provides a 2x magnification and can be used with all Leica 2200 series rotary microtomes. 2 4 Always protect the magnifier glass from exposure to direct sunlight! Exposure to sunlight may cause a burning glass effect.
56 Instruction manual V 1.4 – 02/2007 2 4 5 6 6. Optional accessories 6.9 Cold light source 6.10 Fiber-optic light guide Fig. 62, Leica rotary microtome with installed optional accessories: magnifying lens and fiber-optic light guide Fig. 58 Fig. 60 Fig.
57 Leica RM2245 6.11 Ordering information Fixture for specimen clamps, rigid, silver ............................................................................. 0502 38160 Fixture for specimen clamps, directional, s ilver ...........................
58 Instruction manual V 1.4 – 02/2007 6. Optional accessories EM specimen holder fixture, black .......... ................................... ................................... ....... 0502 29 968 EM univ. specimen holder .........................
59 Leica RM 2245 7. Customized solutions Certain instrument malfunctions appear on the 3-digit LED display or are indicated through illuminated LED’s on the control panel. 7.1.1 Error reports Indication E1 If this message follows the software version indication after switching on the instrument, a loss of all parameters has occurred.
60 Instruction manual V 1.4 – 02/2007 7. Customized solutions • Feed motion no longer occurs. Problem Corrective action Possible cause 1. STEP mode is still active (LED in SECT button lights up). 1. The end of the residual path has been reached. 2.
61 Leica RM 2 2 45 7. Customized solutions 1. Thick/thin sections The sections alternate between being thick and thin. In extreme cases, there are no sections whatsoever. • Check the clamping positions and repeat the clamping process • Laterally displace the knife holder or insert a new blade.
62 Instruction manual V 1.4 – 02/2007 Cleaning the knife Always wipe the knife from the back of the knife to the cutting edge. NEVER wipe in the opposite direction - risk of injury! Clean using an alcohol-based solution or acetone.
63 Leica RM2245 8. Cleaning and maintenance Knife holder E Fig. 61 10 11 9 Fig. 62 86 83 83 86 87 11 10 Take the knife holder apart for cleaning. To do so, proceed as follows: • Fold the knife guard ( 9 ) and ejector ( 84 ) downward. • Rotate the clamping lever ( 11 ) of the lateral dis- placement forwards and pull it out sideways.
64 Instruction manual V 1.4 – 02/2007 Universal cassette clamp • Detach cassette clamp ( 13 ) for a thorough cleaning, removing all paraffin residues. • For cleaning, do not use xylene. Use xylene substitutes or paraffin removers such as "Para Gard".
65 Leica RM2245 8.2.2 Maintenance instructions Only authorized and qualified service personnel may access the internal components of the instrument for service and repair! The instrument is basically maintenance-free. To ensure trouble-free operation of the instrument over a long period of time, the following is recommended by Leica: 3.
66 Instruction manual V 1.4 – 02/2007 8. Cleaning and maintenance 97 88 73 11 10 90 Universal cassette clamp: • Shaft ( 90 ) and flat coil spring ( 91 ) of the clamping lever of the cassette clamp. After lubrication, move the lever back and forth several times.
67 Leica RM 2245 9. Warranty and service Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure bas.
. . . . . . . . . . . . . . . . . . . . . . . . . . . . Dag Graupner Managing Director EC Declaration of Conformity Leica Microsystems Nussloch GmbH Postfach 1120 D-69222 Nussloch January 12, 2004 10.
Een belangrijk punt na aankoop van elk apparaat Leica RM2245 (of zelfs voordat je het koopt) is om de handleiding te lezen. Dit moeten wij doen vanwege een paar simpele redenen:
Als u nog geen Leica RM2245 heb gekocht dan nu is een goed moment om kennis te maken met de basisgegevens van het product. Eerst kijk dan naar de eerste pagina\'s van de handleiding, die je hierboven vindt. Je moet daar de belangrijkste technische gegevens Leica RM2245 vinden. Op dit manier kan je controleren of het apparaat aan jouw behoeften voldoet. Op de volgende pagina's van de handleiding Leica RM2245 leer je over alle kenmerken van het product en krijg je informatie over de werking. De informatie die je over Leica RM2245 krijgt, zal je zeker helpen om een besluit over de aankoop te nemen.
In een situatie waarin je al een beziter van Leica RM2245 bent, maar toch heb je de instructies niet gelezen, moet je het doen voor de hierboven beschreven redenen. Je zult dan weten of je goed de alle beschikbare functies heb gebruikt, en of je fouten heb gemaakt die het leven van de Leica RM2245 kunnen verkorten.
Maar de belangrijkste taak van de handleiding is om de gebruiker bij het oplossen van problemen te helpen met Leica RM2245 . Bijna altijd, zal je daar het vinden Troubleshooting met de meest voorkomende storingen en defecten #MANUAl# samen met de instructies over hun opplosinge. Zelfs als je zelf niet kan om het probleem op te lossen, zal de instructie je de weg wijzen naar verdere andere procedure, bijv. door contact met de klantenservice of het dichtstbijzijnde servicecentrum.
