Gebruiksaanwijzing /service van het product MDS100 van de fabrikant 3M
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EN Molecular Detection System User Manual MDS100.
1 EN (English) T able of C ontents .................................................................. 1 Usi ng th is Man ual ................................................................. 3 Intende d Us e ...........................................
2 EN (English) Performing Runs ................................................................36 Setting up a new run ................................................. 36 Starting a run ............................................................ 40 Importing a new run .
3 EN (English) Using this Manual 1 . The 3M ™ Molecular Detection System User Manual is provided in electronic format only . T o access the user manual, c lick [Help] on the menu bar , and then select User Manual.
4 EN (English) User Responsibility USER RESPONSIBILITY Users are responsible for familiarizing themselves with product instructions and information. Visit our website a t www .3M.com/foodsafety , or contact your local 3M representa tive or distributor for more information.
5 EN (English) W ARNING • T o reduce the risks associated with hazardous voltage and fire: - keep power supply visible and accessible a t all times. Use the plug at the wall socket to disconnect the device from the main power supply . - use only the power cord specified for this product and certified for the countr y of use.
6 EN (English) Instrument Specifications Electrical Specifications Model MDS100 Specification Units Voltage 24 V VDC Current 2.5 A Amps Power Supply Specification Units Input Voltage 100-240 V VA C Frequenc y 50/60 Hz Hertz Maximum Input Current 1.
7 EN (English) Regulator y Informa tion Safety Standards IEC/EN 61010-1, 2 nd Edition UL 61010-1 CAN/CSA C22.2 No. 61010-1 EIC 61010-2-081 EMC Standards USA: FCC part 15 subpart B Canada: ICES-003 EEA: EN 61326 Australia: AS/NZF 2064.
8 EN (English) Minimum Computer Requirements • Microsoft® Windows® XP with Service Pack 3 (SP3), or Microsoft® Windows® Vista with Ser vice Pack 2 (SP2), or Microsoft® Windows® 7 (32- or 64-bit) • 2.
9 EN (English) 8. Use a disposable towel lightly dampened with DI wa ter (distilled or deionized water) to gently wipe any dust or debris from the inner surfaces of the instrument. 9. Use a dr y disposable towel to wipe the interior surfaces of the instrument.
10 EN (English) Installing the Software The 3M Molecular Detection Software comes packaged with Microsoft.NET Framework 4 and Microsoft SQL Server 2008 R2 Express. T o install the 3M Molecular Detection Software, you must be logged onto Windows as an administrator or belong to the Administrators group.
11 EN (English) 7. Read the the Microsoft license terms, and then c lick the I have read and accept the license terms check box to accept the Microsoft license terms and click the [Install] button. 8. After installation is complete, a computer restart may be required.
12 EN (English) 10. When the SQL Ser ver Installation Center opens, select [New installation or add features to an existing installation and click [Ne xt]. . 11. Read and accept the SQL Ser ver license agreement. 12. In the Feature Selection screen, click [Next] to accept the default Instance F eatures, Shared Features, and directory location.
13 EN (English) 14. In the Ser ver Configuration screen, select NT AUTHORITYSYSTEM for the Account Name field for the SQL Server Database Engine. No Password is required. Click [Next] to continue. 15. Click [Next] to accept the default Windo ws authentication mode.
14 EN (English) 19. In the Database Connection Settings screen, the Server Name will be automatically populated. This screen may be blank for a few seconds. Click Next to accept the default settings unless you have a specific reason to edit them. 20.
15 EN (English) 23. Click [Ne xt] to continue with the installation wizard. 24. Select a format for the default Run ID. The available forma ts of the default Run ID all include the current date followed by the creation sequence number . The date format is a two digit month, two digit day and either a two or four digit year .
16 EN (English) Sample Fields Field Name Field T ype Field Values Show Field 1 Sample T ype List Raw Processed Environmental Show Field 2 Description T ext Show Field 3 Product T ext Show Field 4 Brand T ext Show Field 5 Lot Number T ext Show Field 6 Line T ext Show Field 7 Customer T ext Show 27.
17 EN (English) 30. In the Import/Export Options screen, you can select the default loca tions for import and export files. This can also be changed after installation, in the Administra tion>Import and Export Options menu.
18 EN (English) 33. Click [Finish] when the installation wizard displays the Completing the 3M Molecular Detection Software Setup Wizard window . Operating the 3M Molecular Detection Software Before u.
19 EN (English) Using the Start P age 1. The 3M Molecular Detection Software displays the Start Page window after you login successfully . Y ou can select and perform the following operations from the Start Page by clicking the text beside the icons.
20 EN (English) 5. From the Collapsing Sidebar , you can select and perform the following operations by clicking the text beside the icons. • Setup New Run – Create a new Run definition •.
21 EN (English) Help User Manual Display the User Manual About 3M Molecular Detection System Display the About screen Using the Status Bar 1. The 3M Molecular Detection Software displays a status bar a t the bottom of the software’ s main window . This status bar contains three components.
22 EN (English) Instrument Sta tus T ab Field Name Description Instrument Name The instrument name you defined when you added the instrument Instrument Status The instrument’ s current operational sta tus. Refer to the table of operational statuses below.
23 EN (English) Status Light Icon Instrument Status Off (White) The instrument is in Standby state while it waits to start the heaters to perform a test run, and the lid is c losed. OR The instrument is in Diagnose state while it runs a Self-Check, and the lid is c losed.
24 EN (English) Setting up the Administra tive Information Users Users Window Adding users Only administrator level users can add users. 1. Click [Administration] on the menu bar , and then select Users…. 2. The Setup window displays a current list of users on the Users tab in the sequence in which they were added.
25 EN (English) Deleting users Only administrator level users can delete users. 1. Click Administration on the menu bar , and then select Users…. 2. The Setup window displays a list of active users on the Users tab in the sequence in which they were added.
26 EN (English) Instruments Instruments Window Adding instruments The 3M Molecular Detection Software automatically detects instruments that are po wered on and are connected to the computer by a USB cable when the software starts.
27 EN (English) 6. The instrument status bar displays only the first four instruments in the Instruments list. These first four instruments that are displayed may or may not be the four instruments that are currently communica ting with the 3M Molecular Detection Software.
28 EN (English) V iewing instruments Any level user can view users. 1. Click Administration on the menu bar , and then select Instruments…. 2. The Instrument tab on the Administration windo w displays all instruments that have been automa tically detected.
29 EN (English) 6. Y ou can enable a F ield Name to make it appear on the Sample tab in the Setup window or you can disable a Field Name to prevent if from appearing on the Sample tab in the Setup window. a. T o enable or disable a Field Name, simply click the check box in the Enabled column.
30 EN (English) Field Change Effects on Sample Setup T ab Samples Samples Window.
31 EN (English) Adding samples 1. Samples are the food or environmental samples that you are testing for the presence of pa thogen. 2. Click Manage on the menu bar , and then select Samples…. Y ou can also select Manage Samples on the Sidebar , or select the Samples tab if the Manage window is alread y open.
32 EN (English) 3. Click the Sample ID that you want to hide. Use the vertical scroll bar to display additional Samples if necessary . Y ou can press and hold [Shift] while you click another Sample ID to hide both Sample IDs and all Sample IDs between them.
33 EN (English) 5. Click the Import button. If only one import file is present in the import folder , it will automatically import. If multiple files or no files are present, the Open window appears. Navigate to the file you want to import and double click the file name.
34 EN (English) Exporting samples 1. Y ou can export Sample IDs and their corresponding values for the custom fields in the form of delimited data files, e.g., Comma Separated V alues (.CSV) files. Use this feature if the Sample IDs can be imported to a LIMS or to another system from the 3M Molecular Detection System.
35 EN (English) 5. Use the Assay T ype drop-do wn menu to select the type of assay . This field is required. Select one of the four pathogen targets or the Matrix Control as indicated on the kit label: • Matrix Control • Salmonella • E.
36 EN (English) P erforming Runs Setting up a new run 1. Launch the 3M Molecular Detection Software using the icon located on the Desktop. 2. Enter your User Name and Password to log in. 3. T o setup a new run, select File on the menu bar , and then select New Run .
37 EN (English) Y ou can also right-c lick a cell or group of cells to select the Assay type and W ell T ype using the context-sensitive menu. 10. Repeat step 8 and step 9 until you ha ve defined all the assay types and well types for the run. The screenshot belo w is an example of a completed new run definition.
38 EN (English) 13. If you are using a Matrix Control, link the sample to the Matrix Control by assigning the same Sample ID to both the sample and the Matrix Control. This associa tion causes the sample result to be dependent on the Matrix Control result.
39 EN (English) 19. Repeat Steps 11 and 12 to enter the Kit Lot Number and the Sample ID for the remaining wells. Leave any unused wells blank (gray circles). 20. The large icons below the Setup (Grid) pane provide quick access to functions commonly used during the run definition process.
40 EN (English) Starting a run 1. Follow the instructions in the assay kit Product Instructions to perform the assay . Do not place the sample reagent tubes into the speed loader tray until you have completed the lysis step.
41 EN (English) • The data in a sample import file that’ s intended to be imported into a list field must match one of the selections for the list field. F or example, a sample type must be Ra w , Processed, or Environmental or one of the sample types you defined.
42 EN (English) Setup (Grid) Assay T ype Pane Saving a run as a template 1 . Crea te a run template by setting up a run structure using the Setup(Grid) or Setup(List) panes without setting up the details in the Well Details pane.
43 EN (English) Save T emplate Dialog Load T emplate Dialog When a Run is created (loaded) based on a T empla te, all defined wells will be marked as incompletely defined. An example of a Run created from a T empla te is provided in Error! Reference source not found.
44 EN (English) Run Created From T emplate V iewing run results 1 . Y ou can use the Results-Grid view to monitor a run in progress. Results are displayed in real-time. 2 . T o view run results from previous runs, select File on the menu bar , and then select Open Runs .
45 EN (English) Well T ype Well Result Symbol Result Interpretation Sample Positive The sample is presumptive positive for the target pathogen. Sample Negative The sample is negative for the target pa thogen. Sample Inhibited The sample matrix was inhibitor y to the assay .
46 EN (English) 11. Y ou can choose to select and display the graph for any or all of the wells. a. Right-click or Ctrl-c lick an individual well to select and display the graph for tha t well.
47 EN (English) 15. Y ou can select the Results (List) to display the same data as the Results (Grid) but in a list form. The following fields are shown: • Well ID – The well ID that correla tes to the well’ s location on the 96-well grid. • Sample ID – The identifier of the sample used in this test.
48 EN (English) Generating run reports Run Layout Report 1 . Use this report to assist with assay set up. 2 . Select the Setup (Grid) view to print or sa ve the Run Layout, which shows the setup definitions for all wells in the run. 3 . Click the print button to open the print preview in the report viewer .
49 EN (English) 2 . If you cannot find the run your want do delete, you can use Run filters to set the plate filter parameters. All of the filter parameters are optional. a. Select the All dates option to include runs created on any da te, or select the Date range option to include runs created within a range of dates.
50 EN (English) Run Status Description All All runs in the database Completed Runs that are completed Completed (successful) Runs that are successfully completed without Invalid, Inhibited, Inspect, o.
51 EN (English) Generating Mana gement Reports Generating a Search Results report 1 . This report allo ws you to quer y the database for specific results from completed runs.
52 EN (English) Lot Number * Optional field. All lot numbers included T ext Line * Optional field. All lines included T ext Customer * Optional field. All plants included T ext * These fields are customizable during software installation. This table shows the default field names and.
53 EN (English) Filter P arameter Description Default T ype User User who did the test. All users included List Sample ID Sample ID as defined in the Samples tab of the Setup window .
54 EN (English) Filter P arameter Description Default T ype User User who did the test. All users included List Assay T ype T arget pathogen or assay type. All assay types included List 1 . Click the [Generate] button after selecting and entering the filters you want to use.
55 EN (English) 1 . Click the [Generate] button after selecting and entering the filters you want to use. Y ou can also c lick the [Cancel] button to return to the Start Page without generating a report. This quer y must scan a large amount of data, and it could return a large number of results.
56 EN (English) Generating User Reports The Users report is only available to administrator level users. 1 . T o generate a Users report, which lists active users, select Reports on the menu bar , and.
57 EN (English) Generating an Instrument T emperature Log report 1 . T o generate an Instrument T emperature Log report, which lists the instrument’ s temperature at 5 second inter vals during all r.
58 EN (English) T erms and Definitions The following terms and definitions are used throughout this document. CSV Comma Separated V alues – a data file where da tum is separated by a delimiter character , commonly a comma.
59 EN (English) Administrator T asks Backing up the Database 1 . Open Microsoft SQL Ser ver Mana gement Studio (SSMS) and connect to the SQLMDS server .
60 EN (English) 3 . Right-click Mds , select T asks , and then select Back Up… 4 . In the Destina tion section, highlight the back up location listed, and c lick the [Remove] button to clear the default destination.
61 EN (English) 5 . T o add a new back up destination, click the [Add…] button, and then click the […] button in the Select Backup Destination dialog. 6 . Select your backup destination and enter a file name with the .bak extension. Use a unique name for each backup file to easily differentiate them.
62 EN (English) 7 . Confirm tha t the new back up destination is listed in the Destination section, and then click [OK] to start the process. 8 . Progress is sho wn in the lower -left of screen. When the restoration is complete, a “successful” message appears.
63 EN (English) Restoring the Database from a Backup Note: If the database version is older than the software version, restore the database following the instructions below and then reinstall the 3M Molecular Detection Software. Be sure to use the e xisting Mds database on installation.
64 EN (English) 3 . In the T o database: field, type “Mds”. 4 . In the Source for restore section, select From device: and c lick the […] button. 5 . Click the [Add] button, and then select the database backup (*.bak) from your storage location.
65 EN (English) 6 . Check the Restore box and click [OK] to start the process. 7 . Progress is sho wn in the lower -left of screen. When the restoration is complete, a “successful” message appears.
66 EN (English) 8 . In the Object Explorer , expand Databases . The Mds da tabase should be listed. Uninstalling the Software Y ou can uninstall the 3M Molecular Detection Software by using any one of these three methods: All Programs menu, Uninstall.
67 EN (English) 9 . The Uninstall 3M Molecular Detection System Uninstall dialog appears. Y ou have the option to delete or to save the existing 3M Molecular Detection System database. The default is to sa ve the database. T o delete the database, c lick the Delete database checkbox.
68 EN (English) Starting a Self-Check 1 . Power -c ycle the 3M Molecular Detection Instrument, or right-click the instrument icon in the software status bar to display the context-sensitive menu. Y ou can also double-c lick the instrument icon to open the instrument tab.
69 EN (English) Firmware Installation F older 5 . If you are upgrading the firmware for the first time using this computer , you must minimize the UpgradeIt window at this time and install the supporting device drivers. 6 . When the Found New Hardware Wizard appears, select Install from a list or specific location (Advanced) .
70 EN (English) Windows XP Firmware Upgrade Driver Installation (2) Windows XP Firmware Upgrade Driver Installation (3).
71 EN (English) Windows XP Firmware Upgrade Driver Installation (4) 10. After the driver is installed, a reboot is required. 11. After the reboot, open the software, right-click the instrument icon, and select Upgrade firmware . 12. Enter “Y es” or “Y” in the command window as shown in below .
72 EN (English) is restricted to Administrator users. Y ou can start the upgrade by clicking Upgrade firmware in the instrument’ s context- sensitive menu or the [Upgrade Firmware] button in the instrument tab.
73 EN (English) Windows 7/V ista Firmware Upgrade Driver Installation (1) Windows 7/V ista Firmware Upgrade Driver Installation (2).
74 EN (English) Windows 7/V ista Firmware Upgrade Driver Installation (3) Windows 7/V ista Firmware Upgrade Driver Installation (4).
75 EN (English) Windows 7/V ista Firmware Upgrade Driver Installation (5) 12. After the driver is installed, enter “Y es” or “Y” in the command window as shown in below . The new firmware is downloaded to the instrument, and the instrument restarts and begins a Self-Check.
76 EN (English) Cleaning and Decontaminating the Instrument WARNING: F ollow established laborator y practices and wear appropriate Personal Protection Equipment (PPE) when handling the instrument or plates or when cleaning and decontaminating the instrument.
77 EN (English) 8 . Hold the 3M Speed Loader T ray under a stream of tap wa ter from the faucet in a sink in the lab to rinse the 3M Speed Loader T ray . 9 . Set the 3M Speed Loader T ray aside in a spot where it can air dr y for at least one hour . 10.
78 EN (English) 13. Use a dry disposable to wel to wipe the pluggable heater until it’ s dr y . 14. Use a dry disposable to wel to gently press down on each spring pin (1) on the pluggable hea ter . 15. Set aside the pluggable heater in a spot where it can air dr y for at least one hour .
79 EN (English) 3 . Disconnect the po wer supply cord (1) from the AC power outlet (4), then disconnect the power supply (2) from the 3M Molecular Detection System power port (3). 4 . Pack the 3M Molecular Detection instrument in the shipping container in which the loaner 3M Molecular Detection instrument arrived.
80 EN (English) T roubleshooting T roubleshooting the 3M Molecular Detection Instrument Problem Possible Cause Solution Instrument remains in Heat state (orange sta tus light) Core T emp shows - Infi.
81 EN (English) Problem Possible Cause Solution Lid does not stay closed Debris in latch and/or inner shell Clean la tch and/or inner shell Solenoid stuck in open position P ower cyc le the instrument. If failure persists, return instrument for repair .
82 EN (English) Problem Possible Cause Solution “An error occurred during application langua ge initialization. Please check the database connection.
83 EN (English) T roubleshooting 3M Molecular Detection Software Operation Problem Possible Cause Solution Import error stating “Invalid file format” The import file does not have the proper file extension or delimiter type.
84 EN (English) Problem Possible Cause Solution A run failed due to Instrument Disconnection USB cable disconnected Check the instrument USB connection Power loss Check the instrument power The software is not responsive to input or seems frozen A report is currently open in the report viewer .
85 EN (English) Example Reports The following shows examples of 3M Molecular Detection Software reports. Some examples are shortened to remove repetitive information.
86 EN (English) Samples Report.
87 EN (English) Assay Kits Report.
88 EN (English) Completed Runs Report.
89 EN (English) Users Report.
90 EN (English) Instrument Self-Check Report.
91 EN (English) Instrument T emperature Log.
92 EN (English) Audit Log.
93 EN (English) Run Layout.
94 EN (English) Run Report (with graphs enabled).
95 EN (English).
96 EN (English) Example Import/Export F iles Sample Comma Separated V alues (.CSV) File The following is content of a Comma Seperated V alues (.CSV) file that can be used to import Sample definitions. Each line is a new Sample definition. The data in each line is ordered.
97 EN (English) Run Results Comma Separated V alues (.CSV) File The following is an example of Run results exported to a Comma Separated V alues (.CSV) file. The following list associates the position of descriptor fields with their values as specified in the first definition.
98 EN (English) License agreement The following terms and conditions apply to your purchase of the 3M Molecular Detection System and your licensing of the software: 3M END USER LICENSE AGREEMENT 3M .
99 EN (English) ANY IMPLIED W ARRANTY ARISING OUT OF A COURSE OF DEALING OR OF PERFORMANCE, CUSTOM OR USAGE OF TRADE. 3M DOES NOT W ARRANT THA T THE SOFTW ARE WILL FUNCTION ERROR FREE.
100 EN (English) 10. ASSIGNMENT User may not assign this Agreement or any interest therein without the prior , written consent of 3M, and any a ttempted assignment shall be void. 11. COMPLETE AGREEMENT User agrees that this Agreement is the complete agreement between 3M and User relating to its subject.
EN (English) 3M United States 3M Center Bldg. 275-5W-05 St. Paul, MN 55144-1000 USA 1-800-328-6553 3M Canada Post Office Box 5757 London, Ontario N6A 4T1 Canada 1-800-563-2921 3M Europe and MEA 3M Deutschland GmbH Carl-Schurz - Strasse 1 D41453 Neuss/Germany +49-2131-14-3000 3M Latin America 3M Center Bldg.
Een belangrijk punt na aankoop van elk apparaat 3M MDS100 (of zelfs voordat je het koopt) is om de handleiding te lezen. Dit moeten wij doen vanwege een paar simpele redenen:
Als u nog geen 3M MDS100 heb gekocht dan nu is een goed moment om kennis te maken met de basisgegevens van het product. Eerst kijk dan naar de eerste pagina\'s van de handleiding, die je hierboven vindt. Je moet daar de belangrijkste technische gegevens 3M MDS100 vinden. Op dit manier kan je controleren of het apparaat aan jouw behoeften voldoet. Op de volgende pagina's van de handleiding 3M MDS100 leer je over alle kenmerken van het product en krijg je informatie over de werking. De informatie die je over 3M MDS100 krijgt, zal je zeker helpen om een besluit over de aankoop te nemen.
In een situatie waarin je al een beziter van 3M MDS100 bent, maar toch heb je de instructies niet gelezen, moet je het doen voor de hierboven beschreven redenen. Je zult dan weten of je goed de alle beschikbare functies heb gebruikt, en of je fouten heb gemaakt die het leven van de 3M MDS100 kunnen verkorten.
Maar de belangrijkste taak van de handleiding is om de gebruiker bij het oplossen van problemen te helpen met 3M MDS100 . Bijna altijd, zal je daar het vinden Troubleshooting met de meest voorkomende storingen en defecten #MANUAl# samen met de instructies over hun opplosinge. Zelfs als je zelf niet kan om het probleem op te lossen, zal de instructie je de weg wijzen naar verdere andere procedure, bijv. door contact met de klantenservice of het dichtstbijzijnde servicecentrum.
