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Leica CM3050 S Cryostat Instruction Manual Leica CM 3050 S – Cryostat English V1.2– 11/2006 Always keep this manual near the instrument. Read carefully prior to operating the instrument.
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3 Leica CM 3050 S – Cryostat Issued by: Leica Microsystems Nussloch GmbH Heidelberger Str. 17 - 19 D-69226 Nussloch Germany Phone: +49 6224 143-0 Fax: +49 6224 143-200 Internet: http://www.histo-solutions.com Serial No.: .............. .............
4 Instruction Manual V1.2 - 11/2006 Table of Contents 1. Important information ........................................................................................................ ........ 7 1.1 Symbols used in this manual and ....................
5 Leica CM 3050 S – Cryostat Table of Contents 4. Instrument Properties ........................................................................................................ ...... 21 4.1 Overv iew ................................................
6 Instruction Manual V1.2 - 11/2006 Table of contents 6. Troubleshooting, applications tips ....................................................................................... 47 6.1 Displayed error messages .......................................
7 Leica CM 3050 S – Cryostat The chapters of the Leica CM3050 S cryostat instruction manual: Chapter 1 Structure of the manual : • Table of contents • Important information on this manual Chapte.
8 Instruction Manual V1.2 - 11/2006 General This instruction manual includes important instructions and information related to the operating safety and maintenance of the instrument.
9 Leica CM 3050 S – Cryostat 2.1 General information on instrument design and safe handling This instrument has been built and tested in ac- cordance with the following safety regulations on electri.
10 Instruction Manual V1.2 - 11/2006 Instruments with sectioning motor: The sectioning motor is now blocked. All instruments: The message ‘LOCKED’ in the display of control panel 1 indicates that the handwheel has been locked: Unlocking: • To unlock, push locking pin ( 2 ) to the left into position ( 2a ).
11 Leica CM 3050 S – Cryostat 2.2.3 Emergency stop function (instruments with sectioning motor only) The emergency stop is activated via the red emergency stop button in control panel 2 or via the footswitch Activating the emergency stop function • Press emergency stop button or step on footswitch forcefully.
12 Instruction Manual V1.2 - 11/2006 2. Safety 2.3 Safety instructions: handling and operation of the instrument 2.3.1 Transport • To avoid severe damage to the instrument by running it while the compressor oil is dis- placed from its regular position: • Do not tilt the instrument, only transport in an upright position.
13 Leica CM 3050 S – Cryostat 2.3.4 Handling microtome knives/blades • Danger! --> Microtome knives and disposable blades have extremely sharp cutting edges and can cause serious injuries. Therefore: • Handle knives / blades with utmost care.
14 Instruction Manual V1.2 - 11/2006 2. Safety 2.3.9 Infectious/radioactive material • Danger! Use caution when working with potentially infectious specimens: -->Risk of infection! • When worki.
15 Leica CM 3050 S – Cryostat 2. Safety 2.3.11 Removing/reinstalling the microtome • Before removing the microtome: • Switch instrument off. • Unplug from mains. • Place handwheel grip in lowest position and lock. --> When removing the microtome, the specimen head must always be lok- ked in the lowest position.
16 Instruction Manual V1.2 - 11/2006 3.2 Unpacking and installation Unpacking instructions are always located in a transparent protective envelope on the outside of the instrument shipping crate. Make sure to read and follow all safety instructions provided in chapter 2.
17 Leica CM 3050 S – Cryostat 3. Installation 3.3 Standard delivery 1 Cryostat with microtome ............................................................................. 0460 32037 1 Handwheel with marking, antibacterial ..........................
18 Instruction Manual V1.2 - 11/2006 Further accessories: Further accessories which you ordered will be included in the box containing the standard delivery items. Knife holders are delivered with anti-roll guide, knife guard, and a separate instruction manual.
19 Leica CM 3050 S – Cryostat 3.4 Installing/inserting accessories 3.4.1 Installing the handwheel • Insert pin ( 1 ) of the handwheel shaft into hole ( 2 ). • Place spring washer ( 3 ) onto screw ( 4 ) as shown. • Tighten screw ( 4 ) with an Allen key.
20 Instruction Manual V1.2 - 11/2006 3.4.3 The footswitch Function The footswitch performs the same task as the RUN/STOP and RUN/ENABLE keys (activating/ deactivating motorized sectioning / trimming). In addition, the footswitch can be used to acti- vate the emergency stop function.
21 Leica CM 3050 S – Cryostat 4. Instrument Properties 4.1 Overview 1 Cryostat CM3050 S 2 Control panel 1 3 Control panel 2 4 Storage shelf, left 5 Storage shelf, right 6 Rubber mat 7 Mains switch /.
22 Instruction Manual V1.2 - 11/2006 4. Instrument Properties 15 13 14 11 17 11 21 20 19 18 16.
23 Leica CM 3050 S – Cryostat 4. Instrument properties Operating temperature range (ambient temperature): + 18°C to + 40°C. All specifications related to tempe- rature are valid only up to an ambi.
24 Instruction Manual V1.2 - 11/2006 4. Instrument properties Microtome Rotary microtome Section thickness setting 0.5 - 300 μm Overall specimen feed 25 mm Vertical stroke 59 mm Specimen retraction 5.
25 Leica CM 3050 S – Cryostat 5. Operation 5.1 Operating the instrument for the first time Prior to setting up the instrument, make sure to read the instruction manual carefully and in full.
26 Instruction Manual V1.2 - 11/2006 5.2 Switching on/fuses Switching on • To switch on the instrument, place switch ( 1 ) in upper lock-in position. Mains switch / automatic cutout for the instrument: In addition to its function as ON/OFF switch, switch ( 1 ) is also the automatic cutout for the in- strument.
27 Leica CM 3050 S – Cryostat 5.3.1 Key functions in control panel 1 5.3 Control panel 1 ON/OFF key for cryochamber illumination. To select the menu items for setting / changing instrument parameters. In the status indication, pressing the ar- row keys displays the individual current parameters.
28 Instruction Manual V1.2 - 11/2006 5. Operation Meaning of indication: Operator activity: yes no no Acknowledge indication by pressing once briefly. Initialization phase 1 Initialization phase 2 Sof.
29 Leica CM 3050 S – Cryostat 5. Operation Once the instrument has gone through the initial sequence of indications, you can go on to the menu functions to select and/or change instru- ment parameters. If display is locked: Press key until display background lights up.
30 Instruction Manual V1.2 - 11/2006 5. Operation 2. Cryochamber temperature: Press key until menu item ‘Set chamber temperature’ is displayed: --> Continue as described for specimen temperature.
31 Leica CM 3050 S – Cryostat The automatic defrost cycle takes place once every 24 hours at the selected defrost time. 7. Duration of defrost cycle: Press key until menu item ‘Defrost period’ is displayed: • Use defrost keys ( or ) to adjust de- frost time.
32 Instruction Manual V1.2 - 11/2006 5. Operation 5.3.5 Status display Each individual parameter is displayed in a certain area of the display screen. Digits 1 and 2 indicate sectioning stroke positio.
33 Leica CM 3050 S – Cryostat 5. Operation These parameters can be displayed via the ar- row keys. When pressing , the parameters are dis- played in the sequence listed on this page; when pressing , they are displayed in re- verse order. The display can be scrolled contin- uously, i.
34 Instruction Manual V1.2 - 11/2006 5. Operation 5.4 Control panel 2 Functional area A: • Sectioning modes • Sectioning window • Sectioning speed • Emergency stop function Functional area B: .
35 Leica CM 3050 S – Cryostat Start / stop motorized sectioning Sectioning mode 1 - Intermittent stroke: • Simultaneously press and hold RUN/STOP and RUN/ENABLE or depress and hold footswitch. • To stop the sectioning stroke, release the keys or the footswitch: The specimen head stops immediately.
36 Instruction Manual V1.2 - 11/2006 The value has been stored when the LED in the sectioning window key lights up briefly. If the LED flashes, the value has not yet been stored, because the user has .
37 Leica CM 3050 S – Cryostat 5.4.3 Selecting sectioning speed • Sectioning speed is selected via the sliding potentiometer. Speed can be changed while sectioning is in motion.
38 Instruction Manual V1.2 - 11/2006 5. Operation 5.4.5 Coarse feed keys The four coarse feed keys in functional area B function as follows: Coarse feed ‘fast backward’ - Travelling speed 1,000 μm/s • Press key to activate. Specimen head travels backward (= away from knife): a.
39 Leica CM 3050 S – Cryostat 5. Operation Press this key to alternate between the two functions. illuminated: Trimming mode is active off: Sectioning mode is active. Selecting trimming thickness • Activate trimming mode • Select trimming thickness (= sectioning thickness at which trimming is carried out) by pressing or .
40 Instruction Manual V1.2 - 11/2006 5.5 Daily operation of the instrument • Select chamber temperature and, in instru- ments with specimen cooling, specimen temperature according to the type of speci- men to be sectioned (see also chapter 6.3 ‘Reference chart for temperature settings’).
41 Leica CM 3050 S – Cryostat 5.5.2 Freezing specimens onto specimen In instruments with specimen cooling, the 90 ° prism, which is inserted into the specimen head, is an additional option to freeze specimens at maximum temperature . Installing the 90 ° prism • Loosen screw (3) at specimen head.
42 Instruction Manual V1.2 - 11/2006 5. Operation 5.5.4 Inserting specimen discs into the • Lock handwheel in upper position. If a knife has already been inserted into the knife holder, cover knife edge with knife guard! • Loosen clamping screw (3).
43 Leica CM 3050 S – Cryostat 5. Operation Inserting the knife • Insert the knife / disposable blade into the knife holder. • Select appropriate clearance angle at knife holder (settings between 4° and 6° are suit- able for the majority of applications).
44 Instruction Manual V1.2 - 11/2006 5. Operation 5.6 Finishing work 5.6.1 Daily routine steps Remove all specimens from the cry- ochamber and store safely! S e e also corresponding safety instruc- tions in chapter 2.3.7 ‘Defrosting / handling frozen tissue’! • Lock handwheel.
45 Leica CM 3050 S – Cryostat HO ME 5.7 Defrosting The Leica CM3050 S has 3 (instruments without specimen cooling: 2) different defrosting options. - Automatic chamber defrost cycle - Manual chamber defrost cycle - Specimen head defrosting (manual) - instru- ments with specimen cooling only.
46 Instruction Manual V1.2 - 11/2006 SE T T E M P OT - 3 5 ° C SE T T E M P C T - 3 0 ° C - 5. Operation 5.7.2 Manual chamber defrost cycle In addition to the automatic defrost cycle, a manual chamber defrost cycle can be activat- ed when necessary.
47 Leica CM 3050 S – Cryostat 6. Troubleshooting, applications tips 6.1 Displayed error messages The autodiagnosis function of the CM3050 S displays error messages in case of certain mal- functions.
48 Instruction Manual V1.1 02/2000 6. Troubleshooting, applications tips 6.2 Potential problems - causes and remedies Remedies - Eliminate draft sources or change place of installation of the cryostat. - Close sliding window. - Consider wearing a mask.
49 Leica CM 3050 S – Cryostat 6. Troubleshooting, applications tips Problem Sections curl on anti-roll plate. Scraping noise during section- ing stroke and specimen return stroke. Ondulated sections. Chatter marks form during sectioning. Condensation forms on anti-roll plate and knife during cleaning.
50 Instruction Manual V1.1 02/2000 6. Troubleshooting, applications tips Causes - Anti-roll plate protrudes too far beyond the knife edge. Adjust- ment was done in the direction of the knife. - Wrong temperature for the tissue to be sectioned. - Knife profile inappropriate for the specimen to be sectioned.
51 Leica CM 3050 S – Cryostat 6. Troubleshooting, applications tips Problem Sections tear. Inconsistent or insufficient specimen feed. Specimen disc cannot be removed. Cryostat inoperational. Sectioning motor inoperational. No or insufficient refrigerating performance.
52 Instruction Manual V1.1 02/2000 6. Troubleshooting, applications tips 6.3 Reference chart for temperature settings (in minus °C) Tissue 10 - 15 15 - 25 25 - 50 Adrenal Bone marrow ❄ Brain .
53 Leica CM 3050 S – Cryostat 7. Disinfection, cleaning and maintenance 7.1 Safety instructions When disinfecting the instrument, make sure to observe the following safety instructions: • ‘Disinfection and Cleaning’ (Chapter 2.3.10) • ‘Handling microtome knives / blades’ (Chapter 2.
54 Instruction Manual V1.2 - 11/2006 7 Disinfection, cleaning and maintenance 7.3 Disinfection with conventional • Switch the instrument off. • Carefully remove knife / blade as well as spec- imens and accessories from the chamber. • Remove section waste from the chamber and dispose of it.
55 Leica CM 3050 S – Cryostat 7.5 Removing / reinstalling the microtome 7.5.1 Removing the microtome When removing the microtome make sure to observe the following safety instructions: • ‘Removing / reinstalling the microtome’ (Chapter 2.3.11) • ‘Defrosting / handling frozen tissue’ (Chapter 2.
56 Instruction Manual V1.2 - 11/2006 7.5.3 Reinstalling the microtome When reinstalling the microtome make sure to observe the following safety instructions: • ‘Removing / reinstalling the microtome’ (Chapter 2.3.11) • Display message ‘Dry Microtome’ (Chapter 2.
57 Leica CM 3050 S – Cryostat Removal • The lamp (3) is located behind the glare shield (4). • Carefully hold lamp with both of your hands. Slightly pull the lamp downward at the left side to remove it from fastening clip (5). • Pull the lamp towards the left to remove it from its socket (6).
58 Instruction Manual V1.2 - 11/2006 8. Warranty and service Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual prod- uct delivered has been subjected to a comprehensive quality.
59 Leica CM 3050 S – Cryostat An electrohydraulic lifting device for individual working height adjustment is available as an optional accessory for the CM3050 S cryostat series.
60 Instruction Manual V1.2 - 11/2006 9. Appendix 1.4 Installation • Open the shipping box and remove all packing material including the Sty- rofoam pieces. • Take the height adjustment device out of the box. • At the place of installation, place the U-frame on the floor with the open side facing yourself.
61 Leica CM 3050 S – Cryostat The control keys are located on top of the con- trol unit (see photograph). • To lift the instrument, press . • To lower the instrument, press . As long as a button is held, the instrument will go up or down until reaching the upper or lower limit.
62 Instruction Manual V1.2 - 11/2006 2.1 Knife holders Knife-holder CN for reusable microtome knives´w/anti-roll guide, adjustable knife guard and knife support bars for slim and wide knives ............................................. 0419 18649 Knife support bar for knife holder CN, for slim knives .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . Dag Graupner Managing Director We herewith declare, in exclusive responsibility, that the Leica CM3050 S – Cryostat was developed, designed an.
64 Instruction Manual V1.2 - 11/2006 11. Decontamination Certificate (master) Dear Customer, any product which is be returned to Leica Microsystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. This certificate, duly completed, must be placed in the unit prior to packaging or handed over to the service engineer.
65 Leica CM 3050 S – Cryostat 11. Decontamination Certificate (master) Important - to avoid refusal of shipment: Place one copy in the unit prior to packaging or hand it over to the service engineer. Customer as- sumes all responsibility for the immediate return shipment of articles sent to Leica without proper decontamination documentation.
66 Instruction Manual V1.2 - 11/2006.
Een belangrijk punt na aankoop van elk apparaat Leica CM3050 S (of zelfs voordat je het koopt) is om de handleiding te lezen. Dit moeten wij doen vanwege een paar simpele redenen:
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In een situatie waarin je al een beziter van Leica CM3050 S bent, maar toch heb je de instructies niet gelezen, moet je het doen voor de hierboven beschreven redenen. Je zult dan weten of je goed de alle beschikbare functies heb gebruikt, en of je fouten heb gemaakt die het leven van de Leica CM3050 S kunnen verkorten.
Maar de belangrijkste taak van de handleiding is om de gebruiker bij het oplossen van problemen te helpen met Leica CM3050 S . Bijna altijd, zal je daar het vinden Troubleshooting met de meest voorkomende storingen en defecten #MANUAl# samen met de instructies over hun opplosinge. Zelfs als je zelf niet kan om het probleem op te lossen, zal de instructie je de weg wijzen naar verdere andere procedure, bijv. door contact met de klantenservice of het dichtstbijzijnde servicecentrum.
